Protein
|
The Biuret test can be used qualitatively to detect protein and can be used quantitatively with the colorimeter to detect the increasing purple colour of the positive test.
Relatively large quantities of protein need to be present for this test to be effective.
|
 |
|
An alternative test uses the Bradford method and is able to detect microgram amounts of protein. With care this test will permit accurate measurements of protein in different foods,
plant and animal tissues, etc.
|
|
|
Biuret reagent contains 0.75M sodium hydroxide.
THE USE OF EYE PROTECTION MUST BE STRICTLY
ENFORCED
In the event of eye contact flood the eye gently with running water for ten minutes and seek medical attention.
|
 |
|
Bradford reagent stock solution contains phosphoric acid (50%).
Diluted for use the reagent is
irritant.
The dye will stain skin and clothes
|
|
See the pages for the Biuret and Bradford tests.
|