Prepare 0.1M solutions of sodium carbonate (Na2CO3·10H2O) (28.62g/l) and sodium hydrogen carbonate (NaHCO3) (8.4g/l)
Mix these in the volumes shown in the table.
pH
Volume of 0.1M Na2CO3 (cm3)
Volume of 0.1M NaHCO3 (cm3)
at 20°C
at 37°C
9.2
8.8
10
90
9.4
9.1
20
80
9.5
9.4
30
70
9.8
9.5
40
60
9.9
9.7
50
50
10.1
9.9
60
40
10.3
10.1
70
30
10.5
10.3
80
20
10.8
10.6
90
10
Delroy & King, Biochem. J. 39, 245 (1945)
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