Citric acid-Sodium citrate buffer 0.1M

Phosphate free buffer


Prepare a 0.1M solution of citric acid monohydrate, C6H8 O7·H2O (21.01g/l) and a 0.1M solution of trisodium citrate dihydrate, C6H5 O7Na3·2H2O (29.41g/l)
Mix the volumes shown in the table.
    pH 0.1M citric acid (cm3) 0.1M trisodium citrate (cm3)
    3.0 82.0 18.0
    3.2 77.5 22.5
    3.4 73.0 27.0
    3.6 68.5 31.5
    3.8 63.5 36.5
    4.0 59.0 41.0
    4.2 54.0 46.0
    4.4 49.5 50.5
    4.6 44.5 55.5
    4.8 40.0 60.0
    5.0 35.0 65.0
    5.2 30.5 69.5
    5.4 25.5 74.5
    5.6 21.0 79.0
    5.8 16.0 84.0
    6.0 11.5 88.5
    6.2 8.0 92.0

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