Higher Biology (revised C274 12)
UNIT 2 - Metabolism and Survival
1. Metabolism is essential for life
  1. Control of metabolic pathways
    • Enzyme induction experiments such as ONPG and lactose metabolism in E. coli and PGlo experiments.
    Enzyme action
      Experiments on reaction rate with increasing substrate concentration.
    Control of metabolic pathways through the regulation of enzyme action
      Investigate the inhibition of beta-galactosidase by galactose and its reversal by increasing ONPG concentration.
      Experiments on product inhibition with phosphatase.
LACTASE

ENZYMES

PHOSPHATASE

  1. Cellular respiration
    • Citric acid cycle
        Experiments on inhibition of citric acid cycle with malonic acid and DCPIP as an indicator of dehydrogenase activity.
      Electron transport chain
        Experiments with yeast dehydrogenase, eg using resazurin.
RESPIRATION
3. Investigating metabolism in microorganisms
  1. Environmental control of metabolism
    1. Culture conditions
      Investigate the growth of microbes under different cultural and environmental conditions using standard laboratory equipment and simple fermenters.
POPULATION GROWTH
UNIT 3 - Sustainability and Interdependence
  1. The science of food production
    1. Plant growth and productivity
      1. Photosynthesis
      2. Energy capture

          Carry out the Hill reaction.
          Experiments on the synthesis of starch from glucose-1-phosphate by potato phosphorylase.

HILL REACTION

PHOSPHORYLASE