Storage
Stored in a refrigerator at about 4°C the kit will keep for at least four weeks.
Stored in a freezer no colder than -20°C the kit will keep for at least six months.
Contents of the 'ELISA' test kit
The box contains:
- 10 strips of 8 plastic wells
- 20 × small, empty plastic pipettes (one per student)
-
20 × 1.5cm3 centrifuge tubes containing ‘serum’ samples (one per student*)
-
2 × 1.5cm3 centrifuge tubes containing positive ‘serum’ samples labelled with red sticker
(remove before use)
-
1 × bottle containing 30cm3 of ‘wash buffer + detergent’ (dilute 1:20 for use**)
- 10 × sealed pipettes containing enzyme substrate (blue S, one per pair of students)
- 10 × sealed pipettes containing positive control (red +, one per pair of students)
- 10 × sealed pipettes containing negative control (green -, one per pair of students)
- 10 × sealed pipettes containing ‘primary antibody’ (yellow PA, one per pair of students)
- 10 × sealed pipettes containing ‘secondary antibody’ (white SA, one per pair of students)
In addition each pair of students will require the following items not included with the kit:
-
a container (eg 100cm3 beaker) containing about 40cm3 of wash buffer.**
- a pipette for use with the wash buffer
- paper towels
- scissors to cut the tips off the sealed pipettes
- marker pens
*If you are using the kit to simulate the spread of a disease you should substitute one of the unmarked samples with one of the
positive samples labelled with a red sticker. Remove the sticker before use if you do not want the source of the outbreak to be
known until the testing is complete.
** The only preparation needed is to dilute the wash buffer by adding 20cm3 to every
1cm3 of buffer.
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